91 research outputs found
Introduction to Configuration Path Integral Monte Carlo
In low-temperature high-density plasmas quantum effects of the electrons are
becoming increasingly important. This requires the development of new
theoretical and computational tools. Quantum Monte Carlo methods are among the
most successful approaches to first-principle simulations of many-body quantum
systems. In this chapter we present a recently developed method---the
configuration path integral Monte Carlo (CPIMC) method for moderately coupled,
highly degenerate fermions at finite temperatures. It is based on the second
quantization representation of the -particle density operator in a basis of
(anti-)symmetrized -particle states (configurations of occupation numbers)
and allows to tread arbitrary pair interactions in a continuous space.
We give a detailed description of the method and discuss the application to
electrons or, more generally, Coulomb-interacting fermions. As a test case we
consider a few quantum particles in a one-dimensional harmonic trap. Depending
on the coupling parameter (ratio of the interaction energy to kinetic energy),
the method strongly reduces the sign problem as compared to direct path
integral Monte Carlo (DPIMC) simulations in the regime of strong degeneracy
which is of particular importance for dense matter in laser plasmas or compact
stars. In order to provide a self-contained introduction, the chapter includes
a short introduction to Metropolis Monte Carlo methods and the second
quantization of quantum mechanics.Comment: chapter in book "Introduction to Complex Plasmas: Scientific
Challenges and Technological Opportunities", Michael Bonitz, K. Becker, J.
Lopez and H. Thomsen (Eds.) Springer Series "Atomic, Optical and Plasma
Physics", vol. 82, Springer 2014, pp. 153-194 ISBN: 978-3-319-05436-0 (Print)
978-3-319-05437-7 (Online
The nature of NV absorbers at high redshift
We present a study of NV absorption systems at 1.5 < z < 2.5 in the optical
spectra of 19 QSOs. Our analysis includes both absorbers arising from the
intergalactic medium as well as systems in the vicinity of the background
quasar. We construct detailed photoionization models to study the physical
conditions and abundances in the absorbers and to constrain the spectral
hardness of the ionizing radiation. The rate of incidence for intervening NV
components is dN/dz = 3.38 +/- 0.43, corresponding to dN/dX = 1.10 +/- 0.14.
The column density distribution function is fitted by the slope beta = 1.89 +/-
0.22, consistent with measurements for CIV and OVI. The narrow line widths
(b_NV ~ 6 km/s) imply photoionization rather than collisions as dominating
ionization process. The column densities of CIV and NV are correlated but show
different slopes for intervening and associated absorbers, which indicates
different ionizing spectra. Associated systems are found to be more metal-rich,
denser, and more compact than intervening absorbers. This conclusion is
independent of the adopted ionizing radiation. For the intervening NV systems
we find typical values of [C/H] ~ -0.6 and n_H ~ 10^-3.6 cm^-3, and sizes of a
few kpc, while for associated NV absorbers we obtain [C/H] ~ +0.7, n_H ~
10^-2.8 cm^-3, and sizes of several 10 pc. The abundance of nitrogen relative
to carbon [N/C] and alpha-elements like oxygen and silicon [N/alpha] is
correlated with [N/H], indicating the enrichment by secondary nitrogen. The
larger scatter in [N/alpha] in intervening systems suggests an inhomogeneous
enrichment of the IGM. There is an anti-correlation between [N/alpha] and
[alpha/C], which could be used to constrain the initial mass function of the
carbon- and nitrogen-producing stellar population.Comment: accepted by A&A, revised versio
The Index-Based Subgraph Matching Algorithm (ISMA): Fast Subgraph Enumeration in Large Networks Using Optimized Search Trees
Subgraph matching algorithms are designed to find all instances of predefined subgraphs in a large graph or network and play an important role in the discovery and analysis of so-called network motifs, subgraph patterns which occur more often than expected by chance. We present the index-based subgraph matching algorithm (ISMA), a novel tree-based algorithm. ISMA realizes a speedup compared to existing algorithms by carefully selecting the order in which the nodes of a query subgraph are investigated. In order to achieve this, we developed a number of data structures and maximally exploited symmetry characteristics of the subgraph. We compared ISMA to a naive recursive tree-based algorithm and to a number of well-known subgraph matching algorithms. Our algorithm outperforms the other algorithms, especially on large networks and with large query subgraphs. An implementation of ISMA in Java is freely available at http://sourceforge.net/projects/isma
Absorption and Metabolism of cis-9,trans-11-CLA and of Its Oxidation Product 9,11-Furan Fatty Acid by Caco-2 Cells
Furan fatty acids (furan-FA) can be formed by auto-oxidation of conjugated linoleic acids (CLA) and may therefore be ingested when CLA-containing foodstuff is consumed. Due to the presence of a furan ring structure, furan-FA may have toxic properties, however, these substances are toxicologically not well characterized so far. Here we show that 9,11-furan-FA, the oxidation product of the major CLA isomer cis-9,trans-11-CLA (c9,t11-CLA), is not toxic to human intestinal Caco-2 cells up to a level of 100 μM. Oil-Red-O staining indicated that 9,11-furan-FA as well as c9,t11-CLA and linoleic acid are taken up by the cells and stored in the form of triglycerides in lipid droplets. Chemical analysis of total cellular lipids revealed that 9,11-furan-FA is partially elongated probably by the enzymatic activity of cellular fatty acid elongases whereas c9,t11-CLA is partially converted to other isomers such as c9,c11-CLA or t9,t11-CLA. In the case of 9,11-furan-FA, there is no indication for any modification or activation of the furan ring system. From these results, we conclude that 9,11-furan-FA has no properties of toxicological relevance at least for Caco-2 cells which serve as a model for enterocytes of the human small intestine
Linear Optics Simulation of Non-Markovian Quantum Dynamics
The simulation of quantum processes is a key goal for the grand programme
aiming at grounding quantum technologies as the way to explore complex
phenomena that are inaccessible through standard, classical calculators. Some
interesting steps have been performed in this direction and this scenario has
recently been extended to open quantum evolutions, marking the possibility to
investigate important features of the way a quantum system interacts with its
environment. Here we demonstrate experimentally the (non-)Markovianity of a
process where system and environment are coupled through a simulated transverse
Ising model. By engineering the evolution in a fully controlled photonic
quantum simulator, we assess and demonstrate the role that system-environment
correlations have in the emergence of memory effects.Comment: 4+2 pages, 4 figures, RevTeX
Graded structure in sexual definitions: categorizations of having “had sex” and virginity loss among homosexual and heterosexual men and women
Definitions of sexual behavior display a robust hierarchy of agreement regarding whether or not acts should be classed as, for example, sex or virginity loss. The current research offers a theoretical explanation for this hierarchy, proposing that sexual definitions display graded categorical structure, arising from goodness of membership judgments. Moderation of this graded structure is also predicted, with the focus here on how sexual orientation identity affects sexual definitions. A total of 300 18- to 30-year-old participants completed an online survey, rating 18 behaviors for how far each constitutes having “had sex” and virginity loss. Participants fell into one of four groups: heterosexual male or female, gay male or lesbian. The predicted ratings hierarchy emerged, in which bidirectional genital acts were rated significantly higher than unidirectional or nonpenetrative contact, which was in turn rated significantly higher than acts involving no genital contact. Moderation of graded structure was also in line with predictions. Compared to the other groups, the lesbian group significantly upgraded ratings of genital contact that was either unidirectional or nonpenetrative. There was also evidence of upgrading by the gay male sample of anal intercourse ratings. These effects are theorized to reflect group-level variation in experience, contextual perspective, and identity-management. The implications of the findings in relation to previous research are discussed. It is suggested that a graded structure approach can greatly benefit future research into sexual definitions, by permitting variable definitions to be predicted and explained, rather than merely identified
Transcriptional Regulation of Human Dual Specificity Protein Phosphatase 1 (DUSP1) Gene by Glucocorticoids
Background: Glucocorticoids are potent anti-inflammatory agents commonly used to treat inflammatory diseases. They convey signals through the intracellular glucocorticoid receptor (GR), which upon binding to ligands, associates with genomic glucocorticoid response elements (GREs) to regulate transcription of associated genes. One mechanism by which glucocorticoids inhibit inflammation is through induction of the dual specificity phosphatase-1 (DUSP1, a.k.a. mitogen-activated protein kinase phosphatase-1, MKP-1) gene. Methodology/Principal Findings: We found that glucocorticoids rapidly increased transcription of DUSP1 within 10 minutes in A549 human lung adenocarcinoma cells. Using chromatin immunoprecipitation (ChIP) scanning, we located a GR binding region between 21421 and 21118 upstream of the DUSP1 transcription start site. This region is active in a reporter system, and mutagenesis analyses identified a functional GRE located between 21337 and 21323. We found that glucocorticoids increased DNase I hypersensitivity, reduced nucleosome density, and increased histone H3 and H4 acetylation within genomic regions surrounding the GRE. ChIP experiments showed that p300 was recruited to the DUSP1 GRE, and RNA interference experiments demonstrated that reduction of p300 decreased glucocorticoid-stimulated DUSP1 gene expression and histone H3 hyperacetylation. Furthermore, overexpression of p300 potentiated glucocorticoid-stimulated activity of a reporter gene containing the DUSP1 GRE, and this coactivation effect was compromised when the histone acetyltransferase domain was mutated. ChIP-reChIP experiments using GR followed by p300 antibodies showed significant enrichment of the DUSP1 GRE upon glucocorticoid treatment, suggesting that GR and p300 are in the same protein complex recruited to the DUSP1 GRE. Conclusions/Significance: Our studies identified a functional GRE for the DUSP1 gene. Moreover, the transcriptional activation of DUSP1 by glucocorticoids requires p300 and a rapid modification of the chromatin structure surrounding the GRE. Overall, understanding the mechanism of glucocorticoid-induced DUSP1 gene transcription could provide insights into therapeutic approaches against inflammatory diseases. © 2010 Shipp et al
Staphylococcal phenotypes induced by naturally occurring and synthetic membrane-interactive polyphenolic β-lactam resistance modifiers.
Galloyl catechins, in particular (-)-epicatechin gallate (ECg), have the capacity to abrogate β-lactam resistance in methicillin-resistant strains of Staphylococcus aureus (MRSA); they also prevent biofilm formation, reduce the secretion of a large proportion of the exoproteome and induce profound changes to cell morphology. Current evidence suggests that these reversible phenotypic traits result from their intercalation into the bacterial cytoplasmic membrane. We have endeavoured to potentiate the capacity of ECg to modify the MRSA phenotype by stepwise removal of hydroxyl groups from the B-ring pharmacophore and the A:C fused ring system of the naturally occurring molecule. ECg binds rapidly to the membrane, inducing up-regulation of genes responsible for protection against cell wall stress and maintenance of membrane integrity and function. Studies with artificial membranes modelled on the lipid composition of the staphylococcal bilayer indicated that ECg adopts a position deep within the lipid palisade, eliciting major alterations in the thermotropic behaviour of the bilayer. The non-galloylated homolog (-)-epicatechin enhanced ECg-mediated effects by facilitating entry of ECg molecules into the membrane. ECg analogs with unnatural B-ring hydroxylation patterns induced higher levels of gene expression and more profound changes to MRSA membrane fluidity than ECg but adopted a more superficial location within the bilayer. ECg possessed a high affinity for the positively charged staphylococcal membrane and induced changes to the biophysical properties of the bilayer that are likely to account for its capacity to disperse the cell wall biosynthetic machinery responsible for β-lactam resistance. The ability to enhance these properties by chemical modification of ECg raises the possibility that more potent analogs could be developed for clinical evaluation
The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis
Background: Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for the production of recombinant proteins. Their known genome sequences enabled several transcriptomic profiling studies under many different environmental conditions, thus mimicking not only perturbations and adaptations which occur in their natural surroundings, but also in industrial processes. Notably, the majority of such transcriptome analyses were performed using non-engineered strains. In this comparative study, the gene expression profiles of S. cerevisiae and P. pastoris, a Crabtree positive and Crabtree negative yeast, respectively, were analyzed for three different oxygenation conditions (normoxic, oxygen-limited and hypoxic) under recombinant protein producing conditions in chemostat cultivations. Results: The major differences in the transcriptomes of S. cerevisiae and P. pastoris were observed between hypoxic and normoxic conditions, where the availability of oxygen strongly affected ergosterol biosynthesis, central carbon metabolism and stress responses, particularly the unfolded protein response. Steady state conditions under low oxygen set-points seemed to perturb the transcriptome of S. cerevisiae to a much lesser extent than the one of P. pastoris, reflecting the major tolerance of the baker's yeast towards oxygen limitation, and a higher fermentative capacity. Further important differences were related to Fab production, which was not significantly affected by oxygen availability in S. cerevisiae, while a clear productivity increase had been previously reported for hypoxically grown P. pastoris. Conclusions: The effect of three different levels of oxygen availability on the physiology of P. pastoris and S. cerevisiae revealed a very distinct remodelling of the transcriptional program, leading to novel insights into the different adaptive responses of Crabtree negative and positive yeasts to oxygen availability. Moreover, the application of such comparative genomic studies to recombinant hosts grown in different environments might lead to the identification of key factors for efficient protein production
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